Third calcium-modulated rod outer segment membrane guanylate cyclase transduction mechanism
Identifieur interne : 003B12 ( Main/Exploration ); précédent : 003B11; suivant : 003B13Third calcium-modulated rod outer segment membrane guanylate cyclase transduction mechanism
Auteurs : Anuradha Krishnan [États-Unis] ; Rafal M. Goraczniak [États-Unis] ; Teresa Duda [États-Unis] ; Rameshwar K. Sharma [États-Unis]Source :
- Molecular and Cellular Biochemistry [ 0300-8177 ] ; 1998-01-01.
English descriptors
- KwdEn :
Abstract
Abstract: Ca2+-modulated rod outer segment membrane guanylate cyclase (ROS-GC1) has been cloned and reconstituted to show that it is regulated by two processes: one inhibitory, the other stimulatory. The inhibitory process is consistent with its linkage to phototransduction; the physiology of the stimulatory process is probably linked to neuronal transmission. In both regulatory processes, calcium modulation of the cyclase takes place through the calcium binding proteins; guanylate cyclase activating proteins (GCAP1 and GCAP2) in the case of the phototransduction process and calcium-dependent GCAP (CD-GCAP) in the case of the stimulatory process. The cyclase domains involved in the two processes are located at two different sites on the ROS-GC1 intracellular region. The GCAP1-modulated domain resides within the aa 447-730 segment of ROS-GC1 and the CD-GCAP-modulated domain resides within the aa 731-1054 segment. In the present study the GCAP2-dependent Ca2+ modulation of the cyclase activity has been reconstituted using recombinant forms of GCAP2 and ROS-GC1, and its mutants. The results indicate that consistent to phototransduction, GCAP2 at low Ca2+ concentration (10 nM) maximally stimulates the cyclase activity of the wild-type and its mutants: ext- (deleted aa 8-408); kin- (deleted aa 447-730) and hybrid consisting of the ext, transmembrane and kin domains of ANF-RGC and the C-terminal domain, aa 731-1054, of ROS-GC1. In all cases, it inhibits the cyclase activity with an IC50 of about 140 nM. A previous study has shown that under identical conditions the kin- and the hybrid mutant are at best only minimally stimulated. Thus, the GCAP1 and GCAP2 signal transduction mechanisms are different, occurring through different modules of ROS-GC1. These findings also demonstrate that the intracellular region of ROS-GC1 is composed of multiple modules, each designed to mediate a particular calcium-specific signalling pathway.
Url:
DOI: 10.1023/A:1006860018300
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001109
- to stream Istex, to step Curation: 001109
- to stream Istex, to step Checkpoint: 001321
- to stream Main, to step Merge: 003B61
- to stream Main, to step Curation: 003B12
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Third calcium-modulated rod outer segment membrane guanylate cyclase transduction mechanism</title><author><name sortKey="Krishnan, Anuradha" sort="Krishnan, Anuradha" uniqKey="Krishnan A" first="Anuradha" last="Krishnan">Anuradha Krishnan</name></author><author><name sortKey="Goraczniak, Rafal M" sort="Goraczniak, Rafal M" uniqKey="Goraczniak R" first="Rafal M." last="Goraczniak">Rafal M. Goraczniak</name></author><author><name sortKey="Duda, Teresa" sort="Duda, Teresa" uniqKey="Duda T" first="Teresa" last="Duda">Teresa Duda</name></author><author><name sortKey="Sharma, Rameshwar K" sort="Sharma, Rameshwar K" uniqKey="Sharma R" first="Rameshwar K." last="Sharma">Rameshwar K. Sharma</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F0F7DD83ED9DC0D4E2FEE3D5E274BDB5573B8A8A</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1023/A:1006860018300</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-9LWNT7LZ-5/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001109</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001109</idno><idno type="wicri:Area/Istex/Curation">001109</idno><idno type="wicri:Area/Istex/Checkpoint">001321</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001321</idno><idno type="wicri:doubleKey">0300-8177:1998:Krishnan A:third:calcium:modulated</idno><idno type="wicri:Area/Main/Merge">003B61</idno><idno type="wicri:Area/Main/Curation">003B12</idno><idno type="wicri:Area/Main/Exploration">003B12</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Third calcium-modulated rod outer segment membrane guanylate cyclase transduction mechanism</title><author><name sortKey="Krishnan, Anuradha" sort="Krishnan, Anuradha" uniqKey="Krishnan A" first="Anuradha" last="Krishnan">Anuradha Krishnan</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Unit of Regulatory and Molecular Biology, Departments of Cell Biology and Ophthalmology NJMS, University of Medicine and Dentistry of New Jersey, 08084, Stratford, New Jersey</wicri:regionArea><placeName><region type="state">New Jersey</region></placeName></affiliation></author><author><name sortKey="Goraczniak, Rafal M" sort="Goraczniak, Rafal M" uniqKey="Goraczniak R" first="Rafal M." last="Goraczniak">Rafal M. Goraczniak</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Unit of Regulatory and Molecular Biology, Departments of Cell Biology and Ophthalmology NJMS, University of Medicine and Dentistry of New Jersey, 08084, Stratford, New Jersey</wicri:regionArea><placeName><region type="state">New Jersey</region></placeName></affiliation></author><author><name sortKey="Duda, Teresa" sort="Duda, Teresa" uniqKey="Duda T" first="Teresa" last="Duda">Teresa Duda</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Unit of Regulatory and Molecular Biology, Departments of Cell Biology and Ophthalmology NJMS, University of Medicine and Dentistry of New Jersey, 08084, Stratford, New Jersey</wicri:regionArea><placeName><region type="state">New Jersey</region></placeName></affiliation></author><author><name sortKey="Sharma, Rameshwar K" sort="Sharma, Rameshwar K" uniqKey="Sharma R" first="Rameshwar K." last="Sharma">Rameshwar K. Sharma</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Unit of Regulatory and Molecular Biology, Departments of Cell Biology and Ophthalmology NJMS, University of Medicine and Dentistry of New Jersey, 08084, Stratford, New Jersey</wicri:regionArea><placeName><region type="state">New Jersey</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular and Cellular Biochemistry</title><title level="j" type="sub">An International Journal for Chemical Biology in Health and Disease</title><title level="j" type="abbrev">Mol Cell Biochem</title><idno type="ISSN">0300-8177</idno><idno type="eISSN">1573-4919</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Boston</pubPlace><date type="published" when="1998-01-01">1998-01-01</date><biblScope unit="volume">178</biblScope><biblScope unit="issue">1-2</biblScope><biblScope unit="page" from="251">251</biblScope><biblScope unit="page" to="259">259</biblScope></imprint><idno type="ISSN">0300-8177</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-8177</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>activating protein</term><term>calcium modulation</term><term>guanylate cyclase</term><term>phototransduction</term><term>rod outer segment</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Ca2+-modulated rod outer segment membrane guanylate cyclase (ROS-GC1) has been cloned and reconstituted to show that it is regulated by two processes: one inhibitory, the other stimulatory. The inhibitory process is consistent with its linkage to phototransduction; the physiology of the stimulatory process is probably linked to neuronal transmission. In both regulatory processes, calcium modulation of the cyclase takes place through the calcium binding proteins; guanylate cyclase activating proteins (GCAP1 and GCAP2) in the case of the phototransduction process and calcium-dependent GCAP (CD-GCAP) in the case of the stimulatory process. The cyclase domains involved in the two processes are located at two different sites on the ROS-GC1 intracellular region. The GCAP1-modulated domain resides within the aa 447-730 segment of ROS-GC1 and the CD-GCAP-modulated domain resides within the aa 731-1054 segment. In the present study the GCAP2-dependent Ca2+ modulation of the cyclase activity has been reconstituted using recombinant forms of GCAP2 and ROS-GC1, and its mutants. The results indicate that consistent to phototransduction, GCAP2 at low Ca2+ concentration (10 nM) maximally stimulates the cyclase activity of the wild-type and its mutants: ext- (deleted aa 8-408); kin- (deleted aa 447-730) and hybrid consisting of the ext, transmembrane and kin domains of ANF-RGC and the C-terminal domain, aa 731-1054, of ROS-GC1. In all cases, it inhibits the cyclase activity with an IC50 of about 140 nM. A previous study has shown that under identical conditions the kin- and the hybrid mutant are at best only minimally stimulated. Thus, the GCAP1 and GCAP2 signal transduction mechanisms are different, occurring through different modules of ROS-GC1. These findings also demonstrate that the intracellular region of ROS-GC1 is composed of multiple modules, each designed to mediate a particular calcium-specific signalling pathway.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>New Jersey</li></region></list><tree><country name="États-Unis"><region name="New Jersey"><name sortKey="Krishnan, Anuradha" sort="Krishnan, Anuradha" uniqKey="Krishnan A" first="Anuradha" last="Krishnan">Anuradha Krishnan</name></region><name sortKey="Duda, Teresa" sort="Duda, Teresa" uniqKey="Duda T" first="Teresa" last="Duda">Teresa Duda</name><name sortKey="Goraczniak, Rafal M" sort="Goraczniak, Rafal M" uniqKey="Goraczniak R" first="Rafal M." last="Goraczniak">Rafal M. Goraczniak</name><name sortKey="Sharma, Rameshwar K" sort="Sharma, Rameshwar K" uniqKey="Sharma R" first="Rameshwar K." last="Sharma">Rameshwar K. Sharma</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003B12 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003B12 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:F0F7DD83ED9DC0D4E2FEE3D5E274BDB5573B8A8A
|texte= Third calcium-modulated rod outer segment membrane guanylate cyclase transduction mechanism
}}
| This area was generated with Dilib version V0.6.33. |  |